Gas-chromatographic determination of mephenytoin and desmethylmephenytoin, after off-column alkylation

Autor:	V A Raisys, A M Zebelman, S F MacMillan
Rok vydání:	1979
Předmět:	Chromatography Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Alkylation chemistry.chemical_compound Adsorption chemistry medicine Seizure control Mephenytoin Derivatization Desmethylmephenytoin Active metabolite medicine.drug
Zdroj:	Clinical Chemistry. 25:172-175
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/25.1.172
Popis:	We describe a gas-liquid chromatographic method for determining mephenytoin and its active metabolite, desmethylmephenytoin, in human serum. 5-Methyl-5-phenylhydantoin is used as the internal standard. The method involves extraction of the drugs by adsorption onto charcoal and off-column derivatization to their pentyl derivatives. Peak height and concentration are linearly related and the day-to-day CV for therapeutic concentration is about 2 to 6%. No interferences by endogenous compounds or drugs commonly used for seizure control have been encountered.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::55bf5f045d8e74a3d5dd6fd107ac22dc https://doi.org/10.1093/clinchem/25.1.172 Zobrazit plný text záznamu