Does Light Stimulus at Eye Opening of the Developing Rat Influence Retinal Expression of GTP-Binding Protein (Go)?
| Autor: | Tomiko Asano, Masami Oguni, Tomoichi Setogawa, Kanefusa Kato, Haruo Shinohara |
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| Rok vydání: | 1998 |
| Předmět: |
Retina
Eye opening genetic structures G protein Retinal General Medicine Biology Stimulus (physiology) Molecular biology eye diseases Sensory Systems Cell biology Cellular and Molecular Neuroscience Ophthalmology chemistry.chemical_compound medicine.anatomical_structure chemistry medicine sense organs |
| Zdroj: | Ophthalmic Research. 30:84-89 |
| ISSN: | 1423-0259 0030-3747 |
| Popis: | We previously hypothesized that light stimulus at eye opening of rats on postnatal days (P) 13 or 14 has an effect on the expression of GTP-binding proteins (Go in the retina) because the concentration of Goα increased rapidly between P10 and P15. This hypothesis was also supported by the findings that the distribution of Goα in the retina was almost the same as that of adult rats between P10 and P15. In this study, pregnant rats were kept in a dark room after vaginal plugs were identified; they gave birth to their pups in the dark, and their pups were reared by their mothers in the dark. The postnatal rats were sacrificed at P10, P15, P18, P22, P24, P27, and P30. Their retinas were investigated immunochemically and immunohistochemically, using Goα antibody, and these results were compared with those of the rat pups reared normally. Only Goα immunoreactivity in the inner nuclear layer of rats reared in the dark room was weaker than in the controls; the distribution of Goα in the retina did not change, as compared with pups reared in normal conditions. In addition, in pups reared in the dark, Goα increased rapidly from P10 to P15. However, the concentration of Goα in the retina of rat pups reared in the dark was significantly low at P22 (p < 0.01) and P30 (p < 0.05), as compared with pups reared in normal conditions. Although the function of Go in the retina may have something to do with the light stimulus after eye opening, it appeared that the expression of Go was not influenced by light stimulus at eye opening. |
| Databáze: | OpenAIRE |
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