HPLC Determination of the Sugar Compositions of the Glycans on the Cationic Peanut Peroxidase

Autor:	R. B. van Huystee, Yan Sun, Bao Lige
Rok vydání:	1997
Předmět:	Glycan Chromatography Glycosylation biology Chemistry Mannose General Chemistry Xylose Fucose carbohydrates (lipids) chemistry.chemical_compound Biochemistry Galactose biology.protein General Agricultural and Biological Sciences Sugar Peroxidase
Zdroj:	Journal of Agricultural and Food Chemistry. 45:4196-4200
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf970419h
Popis:	Cationic peanut peroxidase has three N-linked glycans. Two glycoforms, CP− and CP+, are known to occur. In this study, the glycans of CP− and CP+ were sequentially separated and identified by trypsin digestion, Bio-Gel P-6 filtration, and reverse phase HPLC. Sugar composition analyses of the glycans were carried out by hydrolysis with TFA, labeling the released sugars with ABEE, and reverse phase HPLC of the ABEE-sugar derivatives. Five different sugars (GlcNAc, Gal, Man, Xyl, Fuc) were found in each of the six glycans investigated. Mannose residues accounted for 31 ± 1.8% of CP− and 40 ± 2.2% of CP+ glycans in term of molar content. The galactose content was 57% lower in CP+ glycans as compared to CP−. The Xyl and Fuc contents were also lower in CP+ than in CP− glycans. A hypothesis made has been confirmed one step further, that is the Gal is one of the terminal sugars of the glycans and the removal of the Gal as a terminal sugar residue in CP− leads to the exposure of Man residue, which is then able to ...
Databáze:	OpenAIRE
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