High resolution ultrathin-layer isoelectric focusing of PGM1-subgroups in forensic blood typing

Autor:	Ulf De La Vigne, Walter Bruder, Werner Pflug
Rok vydání:	1981
Předmět:	Chromatography Chemistry Isoelectric focusing Clinical Biochemistry Blood Stains Polyacrylamide Analytical chemistry High resolution Field strength Biochemistry Blood typing Analytical Chemistry chemistry.chemical_compound PGM1 Layer (electronics)
Zdroj:	Electrophoresis. 2:327-330
ISSN:	1522-2683 0173-0835
DOI:	10.1002/elps.1150020514
Popis:	Ultrathin-layer isoelectric focusing in 100 um polyacrylamide gels is described for the separation of the isozymes of the PGM1-locus. The PGM1-subgroups are best resolved in pH 5–7 gradients obtained by mixing Ampholine and Servalyt carrier ampholytes in a 2:1 ratio. By using a high field strength (180 V/cm) in the final stage of isoelectric focusing, the isozyme bands are sharper than in conventional thin-layer gels. This facilitates identification of the subgroups. Distortions of the pH gradient are avoided if low initial field strengths (15–20 V/cm) are used. The method is highly reproducible and equally applicable for the analysis of blood samples as well as blood stains.
Databáze:	OpenAIRE
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