Practical use of chemical probes for reactive oxygen species produced in biological systems by γ-irradiation

Autor:	Kang-Soo Lee, Jin-Hong Kim, Jae-Sung Kim, Min Hee Lee, Byung Yeoup Chung, Jae-Young Cho, Yu Ran Moon
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Reactive oxygen species Radiation Ethanol biology Chemistry biology.organism_classification γ irradiation In vitro law.invention chemistry.chemical_compound law Spinach Hydroxyl radical Hydrogen peroxide Electron paramagnetic resonance Nuclear chemistry
Zdroj:	Radiation Physics and Chemistry. 78:323-327
ISSN:	0969-806X
DOI:	10.1016/j.radphyschem.2009.03.001
Popis:	Application of chemical probes, for detection of reactive oxygen species (ROS), was tested during γ-irradiation. The ethanol/α-(4-pyridyl-1-oxide)- N - tert -butylnitrone (4-POBN) and 3,3′-diaminobenzidine (DAB) were structurally stable enough to detect OH and H 2 O 2 , increasingly generated by γ-irradiation up to 1000 Gy. Interestingly, the production rate of H 2 O 2 , but not OH, during γ-irradiation, was significantly different between in vitro systems of lettuce and spinach. These results suggest that 4-POBN and DAB could be utilized as a semi-quantitative probe to quantify OH and H 2 O 2 , produced by γ-irradiation up to 1000 Gy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5584d38f0f631e41a526f726ceaba962 https://doi.org/10.1016/j.radphyschem.2009.03.001 Zobrazit plný text záznamu Full Text from ScienceDirect