Development, characterization, molecular docking, and in vivo skin penetration of coenzyme Q10 nanostructured lipid carriers using tristearin and stearyl alcohol for dermal delivery
| Autor: | Nadia Fitria Indah Sari, Ni Luh Dewi Aryani, Siswandono Siswodihardjo, Widji Soeratri |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Coenzyme Q10 Isopropyl palmitate Chromatography Physiology Chemistry Dispersity 02 engineering and technology General Medicine 021001 nanoscience & nanotechnology 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Differential scanning calorimetry Dynamic light scattering Drug Discovery Zeta potential Particle size 0210 nano-technology Stearyl alcohol |
| Zdroj: | Journal of Basic and Clinical Physiology and Pharmacology. 32:517-525 |
| ISSN: | 2191-0286 |
| DOI: | 10.1515/jbcpp-2020-0512 |
| Popis: | Objectives This study aims to develop coenzyme Q10 nanostructured lipid carriers (NLCs) using tristearin and stearyl alcohol as well as isopropyl palmitate (IPP) as solid and liquid lipid respectively for the dermal delivery system. Methods The coenzyme Q10 NLCs were optimized using tristearin, and stearyl alcohol in different concentrations and further characterized by dynamic light scattering (DLS) for particle size, polydispersity index (PDI), zeta potential, differential scanning calorimetry (DSC) and X-ray diffractometry for crystallinity behavior, Fourier transform infrared spectroscopy (FT-IR) for drug-lipid interaction, scanning electron microscopy (SEM) for particle shape, viscometer for viscosity, and pH meter for pH value. Furthermore, entrapment efficiency (EE), drug loading (DL), and skin penetration in vivo were also evaluated while molecular docking was conducted to examine the interaction between coenzyme Q10 and the lipids. Results The coenzyme Q10 NLCs with tristearin-IPP and stearyl alcohol-IPP as lipid matrix had in vivo skin penetration of the NLCs using tristearin were higher compared to stearyl alcohol, however, the skin penetration depths for both NLCs were not significantly different. Furthermore, the in silico binding energy of coenzyme Q10-tristearin was lower compared to coenzyme Q10-stearyl alcohol. Both of them showed hydrophobic and van der Waals interaction. Conclusions The NLCs of coenzyme Q10 were formulated successfully using tristearin-IPP and stearyl alcohol-IPP for dermal delivery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|