A further fractionation of agarose

Autor: R. B. Provonchee, F. H. Kirkpatrick, M. M. Dumais, S. Nochumson, K. B. Guiseley
Rok vydání: 1993
Předmět:
Zdroj: Fourteenth International Seaweed Symposium ISBN: 9789401048828
DOI: 10.1007/978-94-011-1998-6_67
Popis: Commercial agarose can be separated into two components by fractional precipitation using alcohols or glycols. The less soluble component has a much higher gel strength and lower endosmosis than the second fraction which can be recovered by salting out or mixing with additional nonsolvent. The ratio of the two fractions over a range of nonsolvent concentrations varies according to the system used. An unanswered question remains regarding the distribution of the fractions — is it bimodal or continuous? Characterization of the fractions and practical applications of the high gel strength fraction will be discussed.
Databáze: OpenAIRE