Chromosome Dropper Tool: Effect of Slide Angles on Chromosome Spread Quality for Murine Embryonic Stem Cells
| Autor: | Thomas L. Saunders, Li Yu Xing, Elizabeth D. Hughes, Yun Yan Qu |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Journal of Histotechnology. 31:75-79 |
| ISSN: | 2046-0236 0147-8885 |
| DOI: | 10.1179/his.2008.31.2.75 |
| Popis: | Chromosome spreads are routinely prepared for cytogenetic analyses such as chromosome counts, karyotyping, and in situ hybridization. Methods to increase yields of usable chromosome spreads are constantly evaluated. A chromosome dropping tool was devised and used to test the effects of dropping fixed cells onto slides at several angles. The Chromosome Dropper tool (CD) can position slides at angles from 0° to 90°; the dropping height of fixed cells can be adjusted from 0.2 cm to 40 cm; the area where the fixed cells strike the slide is easily selected. CD was tested by making 100 slides of mouse embryonic stem cell chromosome spreads under 25 different dropping angles and height combinations. The quality of 16,699 spreads was evaluated. Angles of 30° or 45° produced more ideal, usable spreads than other angles. The proportion of chromosome spreads that extended beyond one field of view or contained bunched overlapping chromosome was reduced under optimal dropping conditions. Highest yields of idea... |
| Databáze: | OpenAIRE |
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