Cloning and Sequence Analysis of the Gene for Phosphoenolpyruvate Carboxylase from an Extreme Thermophile, Thermus sp1

Autor:	Issei Yoshioka, Mamoru Takahashi, Tsutomu Nakamura, Hiroyuki Toh, Katsura Izui
Rok vydání:	1995
Předmět:	biology Chemistry Sequence analysis Thermus General Medicine Molecular cloning medicine.disease_cause biology.organism_classification Biochemistry Molecular biology Open reading frame medicine Phosphoenolpyruvate carboxylase Molecular Biology Gene Escherichia coli Peptide sequence
Zdroj:	The Journal of Biochemistry. 118:319-324
ISSN:	1756-2651 0021-924X
DOI:	10.1093/oxfordjournals.jbchem.a124909
Popis:	The ppc gene, which encodes phosphoenolpyruvate carboxylase (PEPC) of an extreme thermophile, Thermus sp., was cloned and sequenced. The ppc gene had a high G+C content (69.2%). An open reading frame for a 857-amino-acid polypeptide was found in the gene. The calculated molecular mass was 95,632. The amino acid sequence of Thermus PEPC was 31-37% identical and 52-57% similar to those of 17 PEPCs from mesophilic organisms. No Cys residue was found in the polypeptide, demonstrating that this residue is not essential for the catalytic activity of PEPC. The cloned gene was expressed in Escherichia coli and thermostable PEPC was obtained.
Databáze:	OpenAIRE
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