Stomatal Characterization of Grass Leaves by Four Preparation Techniques

Autor:	John S. Gardner, Val Jo Anderson, Joyce Phillips Hardy
Rok vydání:	1995
Předmět:	biology Scanning electron microscope Cuticle biology.organism_classification chemistry.chemical_compound Horticulture Dactylis glomerata chemistry Freeze substitution Guard cell Air drying Cellular Morphology Instrumentation Tetramethylsilane
Zdroj:	Microscopy and Microanalysis. 1:131-135
ISSN:	1435-8115 1431-9276
Popis:	Four leaf preparation techniques (air drying, tetramethylsilane air drying, critical point drying, and freeze substitution) used in scanning electron microscopy (SEM) were evaluated with respect to the degree of cellular distortion they produce in stomatal guard cells of leaves of Dactylis glomerata and Elymus canadensis. Surface morphological distortion and cuticle disruption in the air-dried and tetramethylsilane air-dried leaves, and cuticle disruption within the critical point-dried tissue made it difficult to obtain measurements.The freeze-substituted tissue experienced little cuticle disturbance, and the cellular morphology appeared normal. The length of the guard cells did not significantly differ between the air-dried, tetramethylsilane air-dried, critical point-dried, or freeze-substituted samples. Widths did significantly vary, with the freeze-substituted tissue having lower values than tissues treated with the other treatments. Freeze substitution methodology produced SEM images that appear to be less distorted and allow easy and precise measurement.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::54ffa425ac15fe269a06f9ead2866c78 https://doi.org/10.1017/s1431927695111319 Zobrazit plný text záznamu