| Popis: |
Most aspects of the cellular effects of leukotriene E 4 (LTE 4 ) on the gene expression in human bronchial epithelial cells (HBEC) are unknown. Cultivated HBEC were exposed to 0, 50 and 100 nM LTE 4 for 48 h. The total RNA was extracted to prepare next-generation sequencing mRNA libraries, sequenced on Illumina HiSeq2500 platform. The results of the differentially expressed genes in response to 50-100 nM LTE 4 were analyzed for biological processes, molecular interactions and significantly influenced pathways. LTE 4 at 50 and 100 nM changed significantly (p 1.5-fold) the expression of 218 and 242 genes, respectively. Of these, expression of 53 genes was altered in response to both 50 and 100 nM LTE 4 . Based on gene ontology, the major groups of the genes were associated with cell proliferation and differentiation (4 genes), cell adhesion (3), receptor activity (3), kinase activity (3), transmembrane transport (3), arachidonic acid metabolism (2), calcium ion binding (3) and nucleic acid binding (10). Among the genes involved in cell proliferation and differentiation, inturned planar cell polarity protein and bone morphogenetic protein-4 were up-regulated, while nuclear factor NF-IL6 and protein kinase D1 were down-regulated. In the cell adhesion group, integrin beta-5 chain was up-regulated, but spondin-2 and fibulin-7 were down-regulated. Among others, Rap guanine nucleotide exchange factor-4 was up-regulated. The results indicate that LTE 4 may significantly influence the performance of HBEC by affecting the expression of genes involved in the inflammatory, remodelling and proliferative responses. Supported by the ESF grants No. 9103 and 9043. |
