New opportunities in the diagnosis of asthma with sensitization to Aspergillus spp
| Autor: | Alexandra E. Uchevatkina, Yana Kozlova, O V Aak, Nikolay Klimko, Ekaterina V. Frolova, Natalya V. Vasilyeva, Larisa Filippova, Valeriy D. Kuznetsov |
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| Rok vydání: | 2021 |
| Předmět: |
Aspergillus
Allergy biology business.industry General Medicine biology.organism_classification Immunoglobulin E medicine.disease Aspergillus fumigatus Basophil activation medicine.anatomical_structure Blood serum Immunology biology.protein Medicine Allergic bronchopulmonary aspergillosis business Sensitization |
| Zdroj: | HERALD of North-Western State Medical University named after I.I. Mechnikov. 13:67-76 |
| ISSN: | 2618-9704 2618-7116 |
| DOI: | 10.17816/mechnikov71585 |
| Popis: | BACKGROUND: Diagnosis of asthma with sensitization to Aspergillus spp. is becoming increasingly important due to the severe, uncontrolled course of the disease and the possibility of the formation of allergic bronchopulmonary aspergillosis. AIM: To evaluate the possibility of using the basophil activation test using flow cytometry for the diagnosis of asthma with sensitization to Aspergillus spp. MATERIALS AND METHODS: 118 patients with asthma were examined. The levels of total IgE and specific IgE to aeroallergens were determined in the blood serum by the enzyme immunoassay. Basophil activation was studied by flow cytometry using the Allergenicity kit (Cellular Analysis of Allergy, Beckman-Coulter, USA). The allergen Aspergillus fumigatus (Alcor Bio, Russia) was used to stimulate basophils. RESULTS: The first group consisted of 57 patients with asthma without sensitization to Aspergillus spp. The second group included 36 patients with asthma with sensitization to Aspergillus spp. The third group consisted of 25 patients with allergic bronchopulmonary aspergillosis. The number of basophils activated by the Aspergillus fumigatus allergen in patients with asthma with sensitization to Aspergillus spp. and allergic bronchopulmonary aspergillosis was significantly higher than in the asthma group and amounted to 8.1 [5.2; 20.9]% and 84.6 [75.7; 94.0]%, respectively ( p < 0.001). The stimulation index in the study groups ranged from 0.7 to 72.6. The optimal diagnostic point (cut off) for identifying patients with asthma with Aspergillus spp. sensitization there was an stimulation index value of more than 2.4, and for patients with allergic bronchopulmonary aspergillosis — 15.95. Among all patients with sensitization to Aspergillus spp. a positive correlation was established between the level of specific IgE to Aspergillus spp. and the percentage of basophils activated by the allergen Aspergillus fumigatus ( r = 0.792, p < 0.001) and stimulation index ( r = 0.796, p < 0.05). CONCLUSIONS: The basophil activation test can be used as an additional diagnostic method for asthma with sensitization to Aspergillus spp. and allergic bronchopulmonary aspergillosis. |
| Databáze: | OpenAIRE |
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