The role of the chondroitin sulfate synthase-1 gene in the immune response of the pearl oyster Pinctada fucata
| Autor: | Yunyan Guan, Hua Zhang, Yu Shi, Maoxian He, Zekui Ou, Xuejie Yi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology Protein domain 04 agricultural and veterinary sciences Aquatic Science biology.organism_classification Cell biology 03 medical and health sciences chemistry.chemical_compound Immune system chemistry Chondroitin sulfate synthase 1 Gene expression 040102 fisheries 0401 agriculture forestry and fisheries Pinctada fucata Chondroitin sulfate Gene Peptide sequence 030304 developmental biology |
| Zdroj: | Fisheries Science. 86:487-494 |
| ISSN: | 1444-2906 0919-9268 |
| DOI: | 10.1007/s12562-020-01420-6 |
| Popis: | Chondroitin sulfate synthase-1 (ChSy-1) plays an important role in the synthesis and chain extension of chondroitin sulfate (CS). CS, a linear polysaccharide with high structural diversity, plays crucial roles in cell adhesion, morphogenesis and cell division in response to viral and bacterial infections. However, the role of ChSy-1 in the immune response remains unknown. In the present study, ChSy-1 from Pinctada fucata was identified. The deduced amino acid sequence of ChSy-1 contains a conserved domain, CHGN, which belongs to glycosyltransferase family A, and multiple sequence alignment revealed that CHGN shows evolutionary differences. Phylogenetic analysis indicated that ChSy-1 may be conserved evolutionarily. The nucleus grafting experiment showed that ChSy-1 possibly participates in wound repair and immune response to the graft. Lipopolysaccharide or polyinosinic:polycytidylic acid challenge and gene expression analysis especially suggested that ChSy-1 is involved in the immune response. The results of the present study indicate that ChSy-1 plays a role in the protection of the pearl oyster against infectious diseases. |
| Databáze: | OpenAIRE |
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