Terminal complement complex formation is associated with intervertebral disc degeneration

Autor: Helena Brisby, Raquel Gonçalves, Rolf E. Brenner, Graciosa Q. Teixeira, Zhiyao Yong, Cornelia Neidlinger-Wilke, Anita Ignatius, Michael Ruf, Uwe Max Mauer, Amelie Kuhn, Jana Riegger, Andreas G. Nerlich, Helena Barreto Henriksson
Rok vydání: 2020
Předmět:
Zdroj: European Spine Journal. 30:217-226
ISSN: 1432-0932
0940-6719
Popis: Purpose The complement system is a crucial part of innate immunity. Recent work demonstrated an unexpected contribution to tissue homeostasis and degeneration. This study investigated for the first time, in human disc tissues, the deposition profile of the complement activation product terminal complement complex (TCC), an inflammatory trigger and inducer of cell lysis, and its inhibitor CD59, and their correlation with the degree of disc degeneration (DD). Methods Disc biopsies were collected from patients diagnosed with DD (n = 39, age 63 ± 12) and adolescent idiopathic scoliosis (AIS, n = 10, age 17 ± 4) and compared with discs from healthy Young (n = 11, age 7 ± 7) and Elder (n = 10, age 65 ± 15) donors. Immunohistochemical detection of TCC and CD59 in nucleus pulposus (NP), annulus fibrosus (AF) and endplate (EP) was correlated with age, Pfirrmann grade and Modic changes. Results Higher percentage of TCC+ cells was detected in the NP and EP of DD compared to Elder (P P P Conclusion TCC deposition positively correlated with the degree of disc degeneration. A functional relevance of TCC may exist in DD, representing a potential target for new therapeutics.
Databáze: OpenAIRE
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