CHEMICAL CHARACTERIZATION, ANTIOXIDANT, CYTOTOXIC, AND ANTIBACTERIAL ACTIVITIES OF EUGENIA UNIFLORA L. AND PSIDIUM CATTLEIANUM SABINE ESSENTIAL OILS AGAINST KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII / CARACTERIZAÇÃO QUÍMICA, ANTIOXIDANTE, CITOTÓXICA E ANTIBACTERIANA DOS ÓLEOS ESSENCIAIS DE EUGENIA UNIFLORA L. E PSIDIUM CATTLEIANUM SABINE SABINE CONTRA KLEBSIELLA PNEUMONIAE E ACINETOBACTER BAUMANNII
| Autor: | Silvia de Oliveira Hübner, Ivandra Ignes de Santi, Rogério Antonio Freitag, Kamila Furtado da Cunha, Suzane Olachea Allend, Mirian Elert da Silva, Marcelle Oliveira Garcia, Daiane D. Hartwig |
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| Rok vydání: | 2021 |
| Předmět: |
Marketing
Pharmacology Organizational Behavior and Human Resource Management Psidium food.ingredient biology Traditional medicine Chemistry DPPH Klebsiella pneumoniae Strategy and Management Eugenia uniflora Pharmaceutical Science biology.organism_classification Acinetobacter baumannii chemistry.chemical_compound Minimum inhibitory concentration food Eucalyptol Drug Discovery Antibacterial activity |
| Zdroj: | Brazilian Journal of Development. 7:27276-27294 |
| ISSN: | 2525-8761 |
| Popis: | INTRODUCTION: Eugenia uniflora L. and Psidium cattleianum Sabine essential oils (EO) can be biologically active and serve as novel sources of antibiotics for Klebsiella pneumoniae and Acinetobacter baumannii bacteria. METHODS: The EO of E. uniflora L. (EOE) and P. cattleianum Sabine (EOP) were extracted from de leaves of the plants and chemically characterized by gas chromatography-mass spectrometry (GC-MS). The antioxidant potential was evaluated by the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) technique and the cytotoxicity evaluated in mammalian VERO cell line by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antibacterial activity of EOE and EOP was assessed by the broth microdilution method. RESULTS: The major compounds of EOE were benzofuran (24.38%), germacrene B (20.12%), β-elemene (9.33%) and β-cubebene (8.55%), and for EOP were α-pinene (24.25%), β-caryophyllene (20.45%), and eucalyptol (10.43%). Both EO tested showed low antioxidant effect and dose-dependent cytotoxicity in VERO cell line. The EOE was less toxic for the mammalian cells with an IC50 (half maximal inhibitory concentration) index of 75.0 mg.mL-1. The EOP and EOE were more active against A. baumannii, with a minimum inhibitory concentration (MIC) of 14.0 and 56.0 mg.mL-1 and a minimal bactericide concentration (MBC) of 14.0 and 112.0 mg.mL-1, respectively. CONCLUSIONS: Although EOE and EOP have low antioxidant and antibacterial activity against A. baumanni and K. pneumoniae new approaches can be applied to improve this effect in vivo. |
| Databáze: | OpenAIRE |
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