Transport and toxicity of 5-fluorouracil, doxorubicin, and cyclophosphamide in in vitro placental barrier model based on BeWo b30 cells

Autor:	D. V. Maltseva, O. V. Kindeeva, V. A. Petrov, G. A. Belyakova, A. Yu. Khristichenko, E N Knyazev, T. N. Gerasimenko, Sergey Nikulin
Rok vydání:	2019
Předmět:	Cyclophosphamide 010405 organic chemistry Chemistry Resazurin General Chemistry Pharmacology 010402 general chemistry 01 natural sciences In vitro 0104 chemical sciences chemistry.chemical_compound Cell culture Toxicity medicine Cytotoxic T cell Doxorubicin Viability assay medicine.drug
Zdroj:	Russian Chemical Bulletin. 68:2344-2349
ISSN:	1573-9171 1066-5285
DOI:	10.1007/s11172-019-2709-7
Popis:	An in vitro placental barrier model based on human choriocarcinoma BeWo b30 cell line was considered as a method of preclinical study of the transport and toxicity of antitumor agents and other organic compounds. Low permeabilities were found for 5-fluorouracil as an example of hydrophilic compound and for doxorubicin as an example of a lipophilic compound with a high degree of binding to proteins and DNA and a high permeability was found for cyclophosphamide as an example of lipophilic compound with a low degree of binding to proteins. Using impedance spectrometry and cell viability assessment via reduction of resazurin to resorufin, a pronounced cytotoxic effect of doxorubicin and good tolerance of 5-fluorouracil and cyclophosphamide by the cells were shown for drug concentrations equal to the maximum concentrations in the patients’ blood during the treatment of breast cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::54ba899b437ce9f7e55244b32bd24f66 https://doi.org/10.1007/s11172-019-2709-7 Zobrazit plný text záznamu Full text from SpringerLink