Characterisation of a ω-gliadin gene in Triticum tauschii
| Autor: | M. C. Gianibelli, M. E. Hassani, Peter Sharp, M. R. Shariflou |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Signal peptide Genetics biology nutritional and metabolic diseases food and beverages Locus (genetics) digestive system Biochemistry digestive system diseases Amino acid chemistry.chemical_compound Protein sequencing chemistry Plant protein biology.protein Gliadin Gene DNA Food Science |
| Zdroj: | Journal of Cereal Science. 47:59-67 |
| ISSN: | 0733-5210 |
| DOI: | 10.1016/j.jcs.2007.01.008 |
| Popis: | A ω-gliadin gene at the Gli-Dt1 locus of Triticum tauschii accession AUS18913 was isolated using PCR primers, designed from published sequences of ω-gliadin genes of bread wheat cv Cheyenne, and deduced sequences of the N-terminal amino acids of ω-gliadin proteins. Further, the derived protein was isolated from A-PAGE and was sequenced. The protein sequence contained a signal peptide of 19 amino acids followed by a short N-terminal sequence of 11 amino acids, a central repetitive domain that covers approximately 90% of the sequence and a short C-terminal domain of 12 amino acids. The sequence comparison with other ω-gliadins showed a high level of similarities between them. Further analysis of the ω-gliadins using A-PAGE revealed that there are three ω-gliadin proteins in AUS18913 accession. Comparison of N-terminal sequences of these proteins revealed that two of these proteins have very high homologies with ω-gliadins of Cheyenne while the third one was significantly different. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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