Aquatic effect duration study of Cry4 toxin with immunoassay andAedes aegyptilarval biotest
| Autor: | Bruce S. Ferguson, Eszter Takács, Béla Darvas, Deepak Saxena, András Székács, Gábor Fekete, Ágnes Fejes |
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| Rok vydání: | 2012 |
| Předmět: |
Active ingredient
Detection limit Larva Chromatography medicine.diagnostic_test Toxin Aedes aegypti Aquatic Science Biology biology.organism_classification medicine.disease_cause Quantitative determination Microbiology Insect Science Immunoassay Bacillus thuringiensis medicine Ecology Evolution Behavior and Systematics |
| Zdroj: | Aquatic Insects. 34:207-222 |
| ISSN: | 1744-4152 0165-0424 |
| DOI: | 10.1080/01650424.2012.643062 |
| Popis: | Bacillus thuringiensis subsp. israelensis (Bti) preparations are widely used for culicid larvae. There is no suitable commercially available analytical method for Cry4 toxin as active ingredient in Bti preparations. To overcome this limitation, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative determination of Cry4 toxin allowing a limit of detection (LOD) of ∼2 ng ml−1 in water. Preconcentration of aqueous samples by lyophilisation resulted in low but reproducible recoveries (25.7±6.8%), and the practical LODs for Bti preparations VECTOBAC WDG granulate and VECTOBAC 12 AS suspension were found to be ∼170 ng ml−1 and ∼900 ng ml−1, respectively. ELISA determinations indicated a rapid decay in detectable concentrations of VECTOBAC WDG applied at 400 ng ml−1 concentration in surface water: detected concentrations decreased by 18% and 44% in 4 days in water collected from two locations, and dropped below LOD afterwards. Larval mortality of Aedes aegypti indicated a continuous decreas... |
| Databáze: | OpenAIRE |
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