Determination of the lipid classes and fatty acid profile of Niger (Guizotia abyssinicaCass.) seed oil
| Autor: | Mohamed Fawzy Ramadan, Jörg-Thomas Mörsel |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Guizotia
chemistry.chemical_classification Chromatography biology Linoleic acid Phospholipid Fatty acid Plant Science General Medicine biology.organism_classification Biochemistry Analytical Chemistry Palmitic acid Oleic acid chemistry.chemical_compound Complementary and alternative medicine chemistry Phosphatidylcholine Drug Discovery Molecular Medicine Organic chemistry lipids (amino acids peptides and proteins) Stearic acid Food Science |
| Zdroj: | Phytochemical Analysis. 14:366-370 |
| ISSN: | 0958-0344 |
| DOI: | 10.1002/pca.729 |
| Popis: | Niger seeds (Guizotia abyssinica Cass.), which are of interest as a new source of vegetable oils, were subjected to Soxhlet-extraction with n-hexane and the extract analysed using a combination of CC, GC, TLC and normal-phase HPLC. The total lipid content was ca. 300 mg/g seed material, and the fatty acid profile showed a high content of linoleic acid (up to 63%) together with palmitic acid (17%), oleic acid (ca. 11%), and stearic acid (ca. 7%). CC separation over silica gel eluted with solvents of increasing polarity yielded 291 mg/g of neutral lipids, 5.76 mg/g of glycolipids, and 0.84 mg/g of phospholipids. GC analysis showed that the major fatty acid present in all lipid classes was linoleic acid together with minor amounts of palmitic, oleic and stearic acids. Polar lipid fractions, however, were characterised by higher levels of palmitic acid and a lower content of linoleic acid. Phospholipid classes separated by normal-phase HPLC consisted of phosphatidylcholine (ca. 49%), phosphatidylethanolamine (22%), phosphatidylinositol (14%), phosphatidylserine (ca. 8%), and minor amounts (2-3%) of phosphatidylglycerol and lysophosphatidylcholine. |
| Databáze: | OpenAIRE |
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