Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

Autor:	Tomáš Gichner, Noemi Cerovska, Petr Vaculik, Tomas Moravec, Helena Plchova
Rok vydání:	2014
Předmět:	Gel electrophoresis biology DNA damage Somatic cell Inoculation Nicotiana tabacum fungi food and beverages Plant Science Horticulture Potato virus X biology.organism_classification Molecular biology Petiole (botany) Comet assay Botany
Zdroj:	Biologia plantarum. 58:783-787
ISSN:	1573-8264 0006-3134
DOI:	10.1007/s10535-014-0448-z
Popis:	We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail moment value, was observed in the inoculated leaves and decreased in the 1st to 4th systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rate of somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5498be80f6e3c9be75b39b7a52275a42 https://doi.org/10.1007/s10535-014-0448-z Zobrazit plný text záznamu Full text from SpringerLink