Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
| Autor: | Mohammad Y. Alshahrani, Amit Kumar Verma, Mohammed Alfaifi, Ali G. Alkhathami, Abdulrahim R. Hakami, Mirza Masroor Ali Beg, Prakash C. Joshi, Irfan Ahmad |
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| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
medicine.medical_specialty business.industry Type 2 Diabetes Mellitus 030209 endocrinology & metabolism Disease 030204 cardiovascular system & hematology medicine.disease Long non-coding RNA Pathogenesis 03 medical and health sciences 0302 clinical medicine Endocrinology Diabetes mellitus Internal medicine Complementary DNA Gene expression microRNA Internal Medicine medicine business |
| Zdroj: | Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 13:3727-3737 |
| ISSN: | 1178-7007 |
| Popis: | Background Type 2 diabetes mellitus [T2DM] has been one of the common diseases and is characterized by increased blood glucose levels and suggested that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in diabetes. Materials/Methods The present study included clinically confirmed newly diagnosed 200 cases of T2DM and 200 healthy subjects, and all the parameters were taken care in diagnosis. Blood samples collected in plain vials were used for cell-free total RNA extraction and after that 100ng of total RNA was used to synthesize the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls. Results It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p 1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p |
| Databáze: | OpenAIRE |
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