Bisdemethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-ĸB, MMP-2 and -9 Pathways
| Autor: | Cheng Yen Chen, An Cheng Huang, Kuang Chi Lai, Ming Jie Hsu, Hui-Yi Lin, Kuo Ching Liu, Yung Lin Chu, Ching Lung Liao, Jing Gung Chung |
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| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
biology Kinase Growth factor medicine.medical_treatment Cell migration General Medicine biology.organism_classification 01 natural sciences In vitro 0104 chemical sciences Blot HeLa 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oncology chemistry Cell culture 030220 oncology & carcinogenesis Bisdemethoxycurcumin medicine Cancer research |
| Zdroj: | Anticancer Research. 38:3989-3997 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | Background/aim Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells. Materials and methods Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro. Results BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERK1/2), E-cadherin and nuclear factor-ĸB (NF-ĸB) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-ĸB when compared to controls. Conclusion BDMC may have potential as a novel anti-metastasis agent for the treatment of human cervical cancer. |
| Databáze: | OpenAIRE |
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