Modulation of luminal L-alanine transport in proximal tubular cells of frog kidney induced by low micromolar Cd2+ concentration
| Autor: | Sanjin Kovacevic, Dusan Cemerikic, Jasna Todorović, Marija Stanojevic, Marko Dinčić, Aleksandar Milovanović, Srdjan Lopicic, Jelena Nesovic-Ostojic, Svetolik Spasic, Marina Savin |
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| Rok vydání: | 2019 |
| Předmět: |
Alanine
Membrane potential Cadmium urogenital system Physiology Health Toxicology and Mutagenesis chemistry.chemical_element Depolarization Cell Biology General Medicine 010501 environmental sciences Toxicology 01 natural sciences Biochemistry Nephrotoxicity 03 medical and health sciences 0302 clinical medicine Membrane chemistry Toxicity Biophysics Repolarization 030212 general & internal medicine 0105 earth and related environmental sciences |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 216:38-42 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2018.11.007 |
| Popis: | The kidneys are recognized as a major target of cadmium-induced toxicity. However, all mechanisms that are involved in the early stages of cadmium nephrotoxicity, particularly considering low micromolar concentrations of cadmium ions (Cd2+) are still unknown. Therefore, the aim of this study was to investigate the effects of peritubular acute exposure to micromolar Cd2+ concentration (2.3 μmol/L) on the rapid depolarization and the rate of slow repolarization of peritubular membrane potential difference (PD), induced by luminal application of L-alanine in proximal tubular cells of frog kidney. The results showed that the luminal application of L-alanine rapidly depolarized the peritubular membrane PD of -42.00 ± 11.68 mV by 23.89 ± 4.15 mV with an average rate of slow repolarization of 5.64 ± 0.81 mV/min. Additionally, peritubular acute exposure to Cd2+ induced change in rapid depolarization of peritubular membrane PD of -53.33 ± 13.01 mV by 18.78 ± 3.31 mV (P < 0.01) after luminal application of L-alanine. Also, peritubular acute exposure to Cd2+ led to statistically significant decrease in the rate of slow repolarization of peritubular membrane PD (3.53 ± 0.35 mV/min; P < 0.05). In conclusion, these results suggest that peritubular acute exposure to low micromolar Cd2+ concentration decreased the rapid depolarization and the rate of slow repolarization of peritubular membrane PD induced by luminal application of L-alanine. This is followed by reduced luminal sodium-coupled transport of L-alanine and this change may be one of the possible mechanisms involved in the early stages of Cd2+-induced nephrotoxicity. |
| Databáze: | OpenAIRE |
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