Differential Regulation of Adaptive and Innate Immunity activated Human Mast Cell by Osteopontin

Autor:	Chun Wai Ng, Hang Yung Alaster Lau
Rok vydání:	2015
Předmět:	Cell type Innate immune system biology Chemistry Mast cell Biochemistry Cell biology chemistry.chemical_compound medicine.anatomical_structure stomatognathic system Immunology Genetics biology.protein medicine Osteopontin Antibody Stem cell Cell adhesion Molecular Biology Histamine Biotechnology
Zdroj:	The FASEB Journal. 29
ISSN:	1530-6860 0892-6638
DOI:	10.1096/fasebj.29.1_supplement.941.12
Popis:	Osteopontin (OPN), a RGD-containing protein which has been shown to enhance inflammatory responses by modulating different functions of different cell types such as macrophages, dendritic cells and natural killer cells. However, OPN has also been shown to suppress inflammatory responses and is important in the healing process. To understand more about OPN, anti-IgE and PGN activated human mast cells developed from human CD34+ stem cells were employed to study the role of this protein in the inflammatory responses. The release of histamine and cytokines (IL-5, IL-8 and TNF-α) releases as well as cell adhesion were studied by spectro-fluorescence assay, ELISA and CyQUANT assay respectively. OPN induced adhesion of human mast cells after anti-IgE and PGN stimulation, and the adhesion was able to inhibit anti-IgE-induced IL-5, IL-8 and TNF-α release while only PGN-induced IL-5 was enhanced by OPN. To investigate the interaction between OPN and human mast cells, cyclic RGD (cRGD) and neutralizing antibodies ag...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::545dcfdc41868eb8e021e17b762453f1 https://doi.org/10.1096/fasebj.29.1_supplement.941.12 Zobrazit plný text záznamu Plný text