Interaction of alcohol and kola nut on brain sodium pump activity in Wistar rats
| Autor: | G.O. Obochi, M.U. Eteng, S.P. Malu, A.E. Abara, I.B. Umoh |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
biology Chemistry Health Toxicology and Mutagenesis ATPase Alcohol Pollution Ouabain Enzyme assay chemistry.chemical_compound Endocrinology Distilled water Biochemistry Internal medicine biology.protein medicine Environmental Chemistry Na+/K+-ATPase Caffeine Ion transporter medicine.drug |
| Zdroj: | Toxicological & Environmental Chemistry. 91:357-362 |
| ISSN: | 1029-0486 0277-2248 |
| Popis: | The effect of interaction of alcohol and kola nut on sodium pump activity was studied in Wistar rats. Thirty Wistar rats were divided into six groups of five rats per group. Control received a placebo (4 mL of distilled water). Groups 2–6 were treated for a period of 21 days with 10% (v/v) alcohol, 50 mg caffeine/kg, 50 mg kola nut/kg, or a combination of 10% (v/v) alcohol + 50 mg kola nut/kg, or 10% (v/v) alcohol + 50 mg caffeine/kg, respectively. One day after the final exposure, brains were harvested and several biochemical parameters examined including activities of total ATPase, ouabain-insensitive ATPase, ouabain sensitive ATPase (Na+–K+-ATPase) and levels of nonenzymatic breakdown of ATP and inorganic phosphate (Pi) released. Results showed that the essential enzyme of the brain responsible for neuronal function, Na+–K+-ATPase, was inhibited by alcohol–kola nut co-administration relative to control, resulting in a decreased ATP production, ion transport and action potential, leading to loss of neur... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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