Sarpeptins A and B, Lipopeptides Produced by Streptomyces sp. KO-7888 Overexpressing a Specific SARP Regulator
| Autor: | Yoko Takahashi, Markiyan Samborskyy, Takuji Nakashima, Kantinan Leetanasaksakul, Arinthip Thamchaipenet, Pakorn Wattana-Amorn, Kazuro Shiomi, Wilaiwan Koomsiri, Yuki Inahashi, Peter F. Leadlay, Satoshi O¯mura |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Regulation of gene expression biology 010405 organic chemistry Chemistry Electrospray ionization Organic Chemistry Pharmaceutical Science Lipopeptide biology.organism_classification 01 natural sciences Streptomyces 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Complementary and alternative medicine Biochemistry Nonribosomal peptide Drug Discovery Gene cluster Molecular Medicine Gene Regulator gene |
| Zdroj: | Journal of Natural Products. 82:2144-2151 |
| ISSN: | 1520-6025 0163-3864 |
| DOI: | 10.1021/acs.jnatprod.9b00074 |
| Popis: | Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed. |
| Databáze: | OpenAIRE |
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