DDX56 inhibits type I interferon by disrupting assembly of IRF3–IPO5 to inhibit IRF3 nucleus import
| Autor: | Shaozu Fu, Hong-Bing Shu, Xiangtao Liu, Yi Ru, Haixue Zheng, Li Dan, Wenping Yang, Zhengqian Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Gene knockdown Innate immune system DEAD box viruses virus diseases Cell Biology biochemical phenomena metabolism and nutrition Biology Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Interferon medicine Nuclear transport IRF3 Transcription factor Nucleus 030304 developmental biology 030215 immunology medicine.drug |
| Zdroj: | Journal of Cell Science. 133 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.230409 |
| Popis: | Transcription factor IRF3-mediated type I interferon induction plays a role in antiviral innate immunity. However, mechanisms for the control and regulation of IRF3 nuclear import remain largely unknown. We have identified DEAD box polypeptide 56 (DDX56) as a negative regulator of virus-triggered IFN-β induction. Overexpression of DDX56 suppressed nuclear translocation of IRF3 via disrupting the IRF3–IOP5 interaction, whereas knockdown or knockout of DDX56 had the opposite effect. In addition, the interaction between DDX56 and IRF3 increased during viral infection. We further found that the D166 site of DDX56 was essential for inhibiting IRF3 import into the nucleus. Our findings suggest that DDX56 regulates antiviral innate immunity by inhibiting the nuclear translocation of IRF3, revealing a novel mechanism of the DDX56-mediated innate antiviral response. This article has an associated First Person interview with the first author of the paper. |
| Databáze: | OpenAIRE |
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