Folic Acid Content of Ready-to-Eat Cereals Determined by Liquid Chromatography-Mass Spectrometry: Comparison to Product Label and to Values Determined by Microbiological Assay
| Autor: | Sungeun Cho, C R. Perry, Katherine M. Phillips, Linda E. Lemar, David M. Ruggio, Joanne M. Holden, Pamela R. Pehrsson, Mehdi Ashraf-Khorassani, Ronald R. Eitenmiller |
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| Rok vydání: | 2010 |
| Předmět: |
Chromatography
Chemistry digestive oral and skin physiology Organic Chemistry Microbiological assay Extraction (chemistry) food and beverages Folic acid Liquid chromatography–mass spectrometry Product Label USDA National Nutrient Database Solid phase extraction Food science Ready to eat cereals Food Science |
| Zdroj: | Cereal Chemistry Journal. 87:42-49 |
| ISSN: | 0009-0352 |
| DOI: | 10.1094/cchem-87-1-0042 |
| Popis: | Twelve popular ready-to-eat breakfast cereals fortified with folic acid were sampled in the United States in 2006, and the data have been incorporated into the USDA National Nutrient Database for Standard Reference. Cereals were collected from three statistically selected retail outlets in each of four primary census regions, and four composites of each product were prepared using random groupings of three locations each. Folic acid was determined using a validated LC-MS method, with 13C-folic acid as an internal standard, after trienzyme treatment and solid phase extraction. A cereal reference material (AACC VMA399) was analyzed as a control. Selected samples were also assayed using the standard microbiological method, with and without trienzyme extraction, to generate an estimate of endogenous folate. On average, as shown on the label, folate content was underestimated. In seven cereals, folate was within 5% of the declared value; in four cereals, it was 5–20% higher; and in two cereals, it was... |
| Databáze: | OpenAIRE |
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