| Popis: |
Rationale Oral antigen delivery with a nonreplicating immunogen has been thought to primarily induce a Th2 response, although high doses of intranasal or systemic antigen delivery with adjuvant create a Th2 response and low doses create a Th1 response. We hypothesized that a similar effect applies to oral antigen delivery. Methods C3H or BALB/c mice were orally immunized weekly for 3 weeks with 2.5 (n=6) or 250μg/dose (n=6) HIV-1 reverse transcriptase (RT) and 1μg cholera toxin (CT) adjuvant or 2, 20, or 200μg recombinant Norwalk virus-like particles (rNV VLP) and 1μg labile toxin LT(R192G) adjuvant, respectively. Mice were sacrificed after two weeks, splenic and mesenteric lymph node mononuclear cells were isolated and serum collected. Results Serum ELISA ratios of IgG1:IgG2a (Th2:Th1) were 1:27 and 1:2 for the 2.5 and 250μg/dose RT groups, respectively (p Conclusions Oral immunization with high or low antigen doses in the presence of adjuvant allows for the selective immunomodulation of Th1 or Th2 responses, respectively, in the systemic and mucosal compartments. |
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