Glycine modulates cytokine secretion, inhibits hepatic damage and improves survival in a model of endotoxemia in mice
| Autor: | Yona Avni, Haim Shirin, Rami Hershkoviz, Rafael Bruck, Joram Wardi, Mark Shahmurov, Ilana Avinoach, Hussein Aeed |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Hepatology Lipopolysaccharide medicine.medical_treatment Intraperitoneal injection Biology Peripheral blood mononuclear cell Amino acid chemistry.chemical_compound Interleukin 10 Cytokine Endocrinology chemistry Internal medicine medicine Cytokine secretion Secretion |
| Zdroj: | Liver International. 23:276-282 |
| ISSN: | 1478-3223 |
| Popis: | BACKGROUND AND AIM There is substantial experimental evidence that the amino acid glycine may have a role in protecting tissues against insults such as ischemia, hypoxia and reperfusion. Our aim was to investigate the ability of the amino acid glycine to prevent hepatic damage induced by injection of lipopolysaccharide and d-galactosamine (d-Gal), to modulate pro- and anti-inflammatory cytokine levels, and to improve survival. METHODS Mice were challenged with an intraperitoneal injection of d-Gal (16 mg/mouse) and lipopolysaccharide (LPS, 1 microg/mouse). The intervention group also received an intraperitoneal injection of glycine (150 mg/kg) in two doses: 24 h before and just after LPS challenge. Serum cytokine levels were measured 2 h after challenge, and liver enzymes and histology were determined 16 h after LPS. Separate groups of mice received the same treatment and the survival rate was determined 24 h and ten days after endotoxin administration. In in vitro experiments, cultured mononuclear cells were stimulated by LPS, and TNF-alpha and IL-10 secretion were measured, in the presence or absence of glycine. RESULTS In the glycine-treated mice, the serum levels of liver enzymes and TNF-alpha, the histologic necroinflammation score and the mortality rate were significantly reduced compared to control mice (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|