Merging Zones FIA System for the Spectrophotometric Determination of Sb(III) and Total Sb in Drugs Used in the Treatment of Leishmaniasis
| Autor: | Vanessa G. K. Almeida, Ricardo J. Cassella, Monica Força Lima |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Flow injection analysis
Bromopyrogallol red Chromatography medicine.diagnostic_test Chemistry Chromogenic Analytical chemistry chemistry.chemical_element Atomic and Molecular Physics and Optics Analytical Chemistry Absorbance chemistry.chemical_compound Antimony Reagent Spectrophotometry medicine Sample preparation Spectroscopy |
| Zdroj: | Spectroscopy Letters. 39:769-784 |
| ISSN: | 1532-2289 0038-7010 |
| DOI: | 10.1080/00387010600970455 |
| Popis: | A merging zones flow injection system was developed for the spectrophotometric determination of Sb(III) and Sb(V) in drugs used in the treatment of leishmaniasis. The procedure is based on the selective reaction between Sb(III) and bromopyrogallol red (BPR) with the decrease of the absorbance measured at 555 nm. The concentration of Sb(V) was calculated by difference after determination of total antimony. Influences of chemical and flow parameters such as pH of reaction, BPR concentration, surfactant concentration, carrier and reagent flow rates, loop volume, and coil reactor length were critically evaluated. Selectivity of the method and the suitable conditions for sample preparation before injection were investigated. The obtained results demonstrated that the addition of Triton X‐100 enhanced the sensitivity of the method due to acceleration of the chromogenic reaction. Working on optimized conditions, the detection (3σ) and quantification (10σ) limits were 30 ng mL−1 and 100 ng mL−1, respecti... |
| Databáze: | OpenAIRE |
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