Use of expressed sequence tag microsatellite markers for population genetic research ofHelicoverpa armigera(Lepidoptera: Noctuidae) from India
| Autor: | Padma Nimmakayala, S. K. Jalali, Gajanan T. Behere, Venkata Gopinath Vajja, V. Sridhar, Ravinder Kumar, Thiruvengadam Venkatesan, Umesh K. Reddy, Rajagopalan M. Shanthi, Yan R. Tomason |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
education.field_of_study Genetic diversity Expressed sequence tag Physiology fungi Population Biology Helicoverpa armigera biology.organism_classification 010603 evolutionary biology 01 natural sciences Gene flow 010602 entomology Structural Biology Evolutionary biology Genetic marker Insect Science Microsatellite PEST analysis education Molecular Biology Ecology Evolution Behavior and Systematics |
| Zdroj: | The Canadian Entomologist. 148:187-199 |
| ISSN: | 1918-3240 0008-347X |
| DOI: | 10.4039/tce.2015.47 |
| Popis: | Cotton bollworm,Helicoverpa armigera(Hübner) (Lepidoptera: Noctuidae), is a serious pest of several crops throughout the world, representing millions of United States of America dollars worth of damage. This pest can adapt to various cropping systems in a wide geographical range and has high migratory potential. It features high fecundity and can develop resistance to almost all insecticides used for its management. Several investigations to develop microsatellite markers forH. armigerahave not been successful because of the paucity of microsatellites in the lepidopteran genome. As well, collections ofH. armigerafrom cotton fields of southern and western India were not yet studied for molecular genetic diversity. The current study aimed to screen publicly available expressed sequence tag resources for simple sequence repeats and assess their potential as DNA markers for assessment of gene flow between collections of southern and western India. We identified 30 polymorphic microsatellites for potential use in diversity analysis ofH. armigeracollections. Genetic diversity analysis revealed that the collections were widely diverse with population differentiation index (Fst) of 0.17. Furthermore, gene flow analysis revealed a mean frequency of private alleles of 11% within the collections. The microsatellite resources we developed could be widely used for molecular diversity or population genetic research involving this important pest of cotton and food crops. |
| Databáze: | OpenAIRE |
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