Immunohistochemical detection of a putative insect cytokine, midgut differentiation factor 1 (MDF-1) in midgut columnar cells ofHeliothis virescens
| Autor: | Shintaro Goto, Makio Takeda, Marcia J. Loeb, Raziel S. Hakim |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Cell type animal structures Heliothis virescens biology fungi Midgut Columnar Cell biology.organism_classification digestive system Molecular biology Endocrinology Manduca sexta Internal medicine parasitic diseases medicine biology.protein Animal Science and Zoology Stem cell Antibody Immunostaining Developmental Biology |
| Zdroj: | Invertebrate Reproduction & Development. 40:117-124 |
| ISSN: | 2157-0272 0792-4259 |
| DOI: | 10.1080/07924259.2001.9652712 |
| Popis: | Summary Two peptides, midgut differentiation factors 1 and 2 (MDF-l and 2), were previously isolated from conditioned medium in which Manduca sexta midgut cells were grown. They are identical to two portions of the terminal sequence of the protein, fetuin, a prenatal vertebrate liver product. In cultures of Lepidopteran midgut, the MDFs act as cytokines or growth factors by inducing midgut stem cells to differentiate to mature columnar, goblet, endocrine, or nerve-like cells. A polyclonal rabbit antiserum to one of these peptides, MDF-l, was used in this study to stain cultured cells from midguts of 4th instar larvae of the Lepidopteran, Heliothis virescens. Paraffin sections of midgut taken from newly molted, mid- 4th instars, and pre-molting 4th instar larvae were also stained with the antibody. Intense positive immunostain was observed in basal areas of the cultured columnar cells and in basal lateral areas of columnar cells in the sections of mid-instar and premolt 4th instar midgut. Other cell types ... |
| Databáze: | OpenAIRE |
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