In Utero and In Vitro Proteinase Activity During the Mesocricetus auratus Embryo Zona Escape Time Window1
| Autor: | Somer A. Mese, Barry D. Bavister, David S. Gonzales |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
biology urogenital system Embryo culture Embryo Cell Biology General Medicine biology.organism_classification Oocyte medicine.anatomical_structure Endocrinology Reproductive Medicine In utero Internal medicine embryonic structures medicine Conceptus Blastocyst Zona pellucida reproductive and urinary physiology Mesocricetus |
| Zdroj: | Biology of Reproduction. 64:222-230 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod64.1.222 |
| Popis: | The goal of the present study was to investigate proteinase activity in uterine flushates collected during the zona loss time window (68–80 h post-egg activation) in both pregnant and pseudopregnant hamsters and in culture medium conditioned by hatching blastocysts. Several prominent enzyme activities appeared in all pregnant and pseudopregnant uterine flushates. However, only a 45, 43 × 10−3 Mr doublet coincided with the zona loss time window; these bands were absent outside of this time window and were not found in conditioned medium. In medium conditioned by hatching blastocysts, enzyme activity was represented by a 70, 65 × 10−3 Mr doublet identical to a doublet seen in all uterine flushates collected and in serum. There were 12 pregnant and 8 pseudopregnant uterine flushates that were capable of zona lytic activity in vitro (positive bioassays). Of these positive bioassays, five pregnant and four pseudopregnant uterine flushates exhibited the 45, 43 × 10−3 Mr doublet (correlative positive bi... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|