Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis
| Autor: | Per Andersson, Wen-Chu Yang, Aroonsiri Shitangkoon, Paul R. Haddad, Philip Zakaria, Pavel N. Nesterenko, Miroslav Macka, Emily F. Hilder |
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| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Chromatography Polytetrafluoroethylene Capillary action Chemistry Organic Chemistry Analytical chemistry General Medicine Polymer Biochemistry Micellar electrokinetic chromatography Analytical Chemistry Electrophoresis chemistry.chemical_compound Crystallinity Capillary electrophoresis Tetrafluoroethylene |
| Zdroj: | Journal of Chromatography A. 1039:193-199 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2004.03.074 |
| Popis: | Poly(tetrafluoroethylene) (PTFE) is a material widely known for its inertness and excellent electrical properties. It is also transparent in the UV region and has a reasonable thermal conductivity. These properties make PTFE a suitable material for the separation capillary in capillary electrophoresis. Differences in the chemistry of the capillary wall compared to fused silica (FS) can make PTFE an interesting alternative to FS for some special applications. In this work, properties of a commercial PTFE capillary of approx. 100 μm i.d. were investigated, including the dependence of electroosmotic flow (EOF) on pH for unmodified and dynamically modified PTFE, optical properties, and practical aspects of use. The main problems encountered for the particular PTFE capillary used in this study were that it was mechanically too soft for routine usage and the crystallinity of the PTFE caused light scattering, leading to high background absorbance values in the low UV region. The profile of the EOF versus pH for bare PTFE surprisingly showed significantly negative EOF values at pH |
| Databáze: | OpenAIRE |
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