Optical Memory Potential of Photoactive Yellow Protein

Autor:	T. Meyer, Galina Khitrova, M. Cusanovich, G. Tollin, H. M. Gibbs
Rok vydání:	1997
Předmět:	biology Thiophenol Kinetics Mutant Chromophore biology.organism_classification medicine.disease_cause Two-photon absorption law.invention chemistry.chemical_compound chemistry Biochemistry law Recombinant DNA medicine Escherichia coli Bacteria
DOI:	10.21236/ada329466
Popis:	This contract targeted improved growth of photoactive yellow protein (PYP) and evaluation of its optical signal processing capabilities. The growth experiments were highly successful. Ectothiorhodopsin halophila bacteria were grown and plenty of PYP isolated for the optical experiments. Then recombinant wild-type aop-protein was grown in E. coli and purified; the chromophore was activated as the thiophenol ester which is very reactive with apo-protein. The reconstituted protein has properties indistinguishable from native protein. Much larger quantities of reconstituted recombinant protein can now be made in E. coli in a shorter period of time than when native protein was prepared in E. halophila. This success has made it possible to make mutants and proteins with variant chromophores, resulting in shifts in the absorption maximum and changes in the photocycle kinetics. These studies are at an early stage and should eventually lead to protein tailored to meet the requirements of photochemical device.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::53b766d964f5163ff7f122039095f24a https://doi.org/10.21236/ada329466 Zobrazit plný text záznamu