Spectroscopic Studies on the Interaction of Naphthyridines with DNA and Fluorescent Detection of DNA in Agarose Gel
| Autor: | P. Lakshmana Rao, Balaraman Selvakumar, M. Seenivasaperumal, Kuppanagounder P. Elango, G. Mahalakshmi, S. Madhuri, K.N. Vennila |
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| Rok vydání: | 2021 |
| Předmět: |
Gel electrophoresis
Circular dichroism Quenching (fluorescence) Sociology and Political Science 010405 organic chemistry Clinical Biochemistry 010402 general chemistry 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Clinical Psychology chemistry.chemical_compound chemistry Biophysics Agarose Ethidium bromide Law Spectroscopy Social Sciences (miscellaneous) Boronic acid DNA |
| Zdroj: | Journal of Fluorescence. 31:327-338 |
| ISSN: | 1573-4994 1053-0509 |
| DOI: | 10.1007/s10895-020-02658-0 |
| Popis: | Four new naphthyridine derivatives (R1-R4) possessing amino acid or boronic acid moieties have been synthesized and characterized using 1H and 13C NMR, FT-IR, and mass spectral techniques. The mechanism of binding of these probes with calf thymus DNA (CT-DNA) has been delineated through UV-Vis, fluorescence, and circular dichroism (CD) spectral techniques along with thermodynamic and molecular docking studies. Small hypochromicity in absorption maximum of the probes without any shift in wavelength of absorption suggests groove binding mode of interaction of these probes with CT-DNA, confirmed by CD and 1H NMR spectral data competitive binding assay with ethidium bromide (EB). CT-DNA quenches the fluorescence of these probes via a static quenching mechanism. In the case of R1 and R4, the observed ΔHo 0suggest that these probes interact with CT-DNA through H-bonding and hydrophobic interactions, while in the interaction of R2 and R3, van der Walls and H-boding forces are found to be dominant (ΔHo |
| Databáze: | OpenAIRE |
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