Mechanism of Sequence-Specific Fluorescent Detection of DNA by N-Methyl-imidazole, N-Methyl-pyrrole, and β-Alanine Linked Polyamides

Autor:	Victor C. Rucker, Harry B. Gray, Peter B. Dervan, Alexander R. Dunn, Shantanu Sharma
Rok vydání:	2004
Předmět:	Alanine Stereochemistry Photochemistry Fluorescence Surfaces Coatings and Films chemistry.chemical_compound Electron transfer chemistry Polyamide Materials Chemistry Imidazole Moiety Physical and Theoretical Chemistry DNA Pyrrole
Zdroj:	The Journal of Physical Chemistry B. 108:7490-7494
ISSN:	1520-5207 1520-6106
DOI:	10.1021/jp037423i
Popis:	The fluorescence from the tetramethylrhodamine (TMR) moiety in hairpin polyamide−TMR conjugates is quenched in solution, but restored upon sequence-specific binding to doubled-stranded DNA. This fluorescence amplification when bound to the target DNA sequence makes polyamide−TMR conjugates potentially useful for the detection of specific DNA sequences in homogeneous solution. Time-resolved and steady-state spectroscopic measurements indicate that a ground-state complex forms between the TMR and polyamide functionalities in the absence of DNA. This intramolecular complex likely facilitates electron transfer from the polyamide N-methyl-pyrrole moieties to the TMR excited state, quenching fluorescence. Binding of the polyamide−TMR probe to the target DNA sequence disrupts the TMR−polyamide interaction, resulting in the observed fluorescence increase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5377e89d1ef1905a7dffe8a9c0686726 https://doi.org/10.1021/jp037423i Zobrazit plný text záznamu