| Autor: |
Nada Townsi, Ingrid A. Laing, Glenys Chidlow, Shannon J. Simpson, Graham L. Hall, David J. Smith, Andrew Wilson, J. Jane Pillow |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Paediatric Respiratory Infection and Immunology. |
| DOI: |
10.1183/1393003.congress-2017.oa4633 |
| Popis: |
Introduction: Longitudinal, population-based studies are essential to assess the burden of respiratory viruses, particularly in children at high risk of respiratory disease. Parental sample collection is feasible, but extended postage times may affect sample quality. Aims and objectives: To assess the effect of environmental exposure on detecting respiratory viruses and pathogenic bacteria in nasal specimens collected from preterm infants. We hypothesised that prolonged environmental exposure would not affect viral detection, but that pathogenic bacterial frequency would be higher. Method: Two consecutive nasal swabs were collected from each infant close to the time of discharge from their birth hospitalisation. One swab was stored immediately at -80°C, and the other was randomised into four groups. Each group was exposed to ambient, non-air conditioned room temperature for 3, 5, 7 or 14 days to simulate the postage environment. Local relative humidity and ambient temperature were obtained daily from the Bureau of Meteorology. Specimens were assessed for the detection of 12 viral and 7 bacterial nucleic acids using PCR. Results: Mean daily temperature and relative humidity were 21.3oC and 80.7%, respectively. Of the 760 molecular assays completed from 20 infants, one tested positive for a respiratory virus (parainfluenza type I). The frequency of detecting pathogenic bacteria was 65% for Staphylococcus aureus and 5% for Streptococcus Pneumoniae . Environmental exposure for up to 14 days did not affect the frequency of detecting respiratory viruses or pathogenic bacteria. Conclusion: This study supports the use of postage of nasal specimens for assessment of respiratory viruses and bacteria in the community. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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