Shiga Toxin Subtypes and Virulence Genes in Escherichia coli Isolated from Cattle
| Autor: | Kenichi Ogita, Yumi Akiyama, Etsuko Saito, Masatsugu Chikahira, Atsushi Iguchi, Hiroko Futai, Hidetaka Tsuji, Hiroshi Sakae, Masaharu Fukunaga |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serotype biology 030106 microbiology Virulence Shiga toxin General Medicine medicine.disease_cause DNA sequencing Microbiology Bacterial adhesin 03 medical and health sciences fluids and secretions 030104 developmental biology Infectious Diseases STX2 biology.protein medicine Gene Escherichia coli |
| Zdroj: | Japanese Journal of Infectious Diseases. 70:181-185 |
| ISSN: | 1884-2836 1344-6304 |
| Popis: | Subtypes of stx1 and stx2 in 45 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle were investigated by PCR. Only subtype stx1a was detected among all the stx1-positive strains. The major stx2 subtype was stx2a followed by stx2d, stx2c, stx2b, and stx2g in decreasing order of frequency. stx2c was found in strains of serotypes O157 and O174. stx2d was found in 11 strains. These strains were confirmed by DNA sequencing to carry both the activatable tail and the END motif; all were eae-negative, and 3 contained stx2d as the only stx. stx2g was found in 2 strains in association with stx2a, estA1, and astA. In addition, 7 hybrid strains of shigatoxigenic and enterotoxigenic E. coli (STEC/ETEC) were found to harbor one or both of stx1a and stx2a (stx1a/stx2a) and estA1. Among 27 serotypes of STEC strains isolated from cattle, O157:H7 and O109:H- strains were eae-positive. Other putative adhesin genes, such as saa, iha, espP, and lpfAO113 were detected in more than 12 serotypes. |
| Databáze: | OpenAIRE |
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