Effect of CdS nanoparticles on the properties of a protein matrix
| Autor: | O. D. Bekasova, A. L. Rusanov, Natalia A. Chebotareva, I.V. Safenkova, Boris I. Kurganov |
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| Rok vydání: | 2011 |
| Předmět: |
Chemistry
General Chemical Engineering Metals and Alloys Analytical chemistry Nanoparticle macromolecular substances Photochemistry Fluorescence Fluorescence spectroscopy Inorganic Chemistry Sedimentation coefficient Materials Chemistry Fluorescence microscope Fluorescence cross-correlation spectroscopy Absorption (chemistry) Laser-induced fluorescence |
| Zdroj: | Inorganic Materials. 47:830-836 |
| ISSN: | 1608-3172 0020-1685 |
| DOI: | 10.1134/s0020168511080024 |
| Popis: | The effect of CdS nanoparticles on the properties of a protein matrix, R-phycoerythrin, has been studied using absorption and fluorescence spectroscopy techniques, atomic force microscopy, confocal fluorescence microscopy, and analytical ultracentrifugation. The results demonstrate that CdS nanoparticles distort the native state of R-phycoerythrin, as evidenced by the reduction in absorption, partial fluorescence quenching, the reduction in fluorescence lifetime from 2.9 to 1.9 ns in solution and the increase to 3.3 ns upon adsorption on glass, and the influence of CdS nanoparticles on the sedimentation coefficient distribution of R-phycoerythrin. We conclude that a CdS nanoparticle synthesized inside an R-phycoerythrin molecule influences the chromophore—chromophore interaction and stabilizes the hexamer form of the protein. |
| Databáze: | OpenAIRE |
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