Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller’s Sea Cow (Hydrodamalis gigas)
| Autor: | Michael G. Harpole, Lorelei Crerar, Jeffery F. Warner |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA education.field_of_study food.ingredient Dugong biology Ecology Population Population genetics Zoology Hydrodamalis biology.organism_classification Steller's sea cow 010603 evolutionary biology 01 natural sciences 03 medical and health sciences 030104 developmental biology food biology.animal Manatee Microsatellite education |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493969890 |
| Popis: | Microsatellite DNA can provide more detailed population genetic information than mitochondrial DNA which is normally used to research ancient bone. The methods detailed in this chapter can be utilized for any type of bone. However, for this example, four microsatellite loci were isolated from Steller's sea cow (Hydrodamalis gigas) using published primers for manatee and dugong microsatellites. The primers DduC05 (Broderick et al., Mol Ecol Notes 6:1275-1277, 2007), Tmakb60, TmaSC5 (Pause et al., Mol Ecol Notes 6: 1073-1076, 2007), and TmaE11 (Garcia-Rodriguez et al., Mol Ecol 12:2161-2163, 2000) all successfully amplified microsatellites from H. gigas. The DNA samples were from bone collected on Bering or St. Lawrence Islands. DNA was analyzed using primers with the fluorescent label FAM-6. Sequenced alleles were then used to indicate a difference in the number of repeats and thus a difference in individuals. This is the first time that H. gigas microsatellite loci have been isolated. These techniques for ancient bone microsatellite analysis allow an estimate of population size for a newly discovered St. Lawrence Island sea cow population. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|