Accumulation of Indole Alkaloids in a Suspension Culture ofTabernaemontana divaricata1
| Autor: | Robert Verpoorte, P. J. Lamping, A. Hermans-Lokkerbol, P. A. A. Harkes, L. P. J. de Kool, R. van der Heijden |
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| Rok vydání: | 1988 |
| Předmět: |
Pharmacology
Indole test Apparicine Chromatography biology Apocynaceae Chemistry Alkaloid Organic Chemistry Tabernaemontana divaricata Pharmaceutical Science Coronaridine biology.organism_classification Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Complementary and alternative medicine Cell culture Drug Discovery Molecular Medicine |
| Zdroj: | Planta Medica. 54:393-397 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-2006-962479 |
| Popis: | Cell suspension cultures of TABERNAEMONTANA DIVARICATA were found to produce relatively large amounts of indole alkaloids. For their isolation an ion-pair DCCC method was used in combination with preparative TLC. The alkaloids were identified as tabernaemontanine, perivine, vobasine, voaphylline, voaphylline-hydroxyindolenine, vallesamine, apparicine, 16-hydroxy-16,22-dihydroapparicine, pericyclivine, tubotaiwine, 19- S-heyneanine, and coronaridine. Voaphylline, the main alkaloid, was produced during growth and early stationary phase and reached a maximum of 23 mg/l at day 19 of the growth cycle. After this maximum voaphylline was rapidly metabolized. Apparicine, vobasine, and coronaridine reached their maximum levels at a later stage of the growth cycle. Tubotaiwine accumulation showed a similar profile as that of voaphylline. In light-grown cells the total production was about 2 times higher than in dark-grown cells, with respective main products voaphylline and apparicine. |
| Databáze: | OpenAIRE |
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