The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway
| Autor: | Hui Zhong, Longquan Shao, Yanli Zhang, Ruolan Wang, Wenjing Liu, Haiyun Luo, Guilan Zhang, Aijie Chen, Liangjiao Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Cell
Biophysics Pharmaceutical Science Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Bone morphogenetic protein 2 Biomaterials Extracellular matrix Downregulation and upregulation Drug Discovery medicine Bone regeneration Chemistry Organic Chemistry technology industry and agriculture General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology RUNX2 medicine.anatomical_structure embryonic structures Alkaline phosphatase Signal transduction 0210 nano-technology |
| Zdroj: | International Journal of Nanomedicine. 15:2419-2435 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s245174 |
| Popis: | Background In recent years, nanomaterials have been increasingly developed and applied in the field of bone tissue engineering. However, there are few studies on the induction of bone regeneration by tantalum nanoparticles (Ta NPs) and no reports on the effects of Ta NPs on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and the underlying mechanisms. The main purpose of this study was to investigate the effects of Ta NPs on bone regeneration and BMSC osteogenic differentiation and the underlying mechanisms. Materials and methods The effects of Ta NPs on bone regeneration were evaluated in an animal experiment, and the effects of Ta NPs on osteogenic differentiation of BMSCs and the underlying mechanisms were evaluated in cell experiments. In the animal experiment, hematoxylin-eosin (HE) staining and hard-tissue section analysis showed that Ta NPs promoted bone regeneration, and immunohistochemistry revealed elevated expression of BMP2 and Smad4 in cells cultured with Ta NPs. Results The results of the cell experiments showed that Ta NPs promoted BMSC proliferation, alkaline phosphatase (ALP) activity, BMP2 secretion and extracellular matrix (ECM) mineralization, and the expression of related osteogenic genes and proteins (especially BMP2, Smad4 and Runx2) was upregulated under culture with Ta NPs. Smad4 expression, ALP activity, ECM mineralization, and osteogenesis-related gene and protein expression decreased after inhibiting Smad4. Conclusion These data suggest that Ta NPs have an osteogenic effect and induce bone regeneration by activating the BMP2/Smad4/Runx2 signaling pathway, which in turn causes BMSCs to undergo osteogenic differentiation. This study provides insight into the molecular mechanisms underlying the effects of Ta NPs in bone regeneration. |
| Databáze: | OpenAIRE |
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