Drug delivery studies in Caco-2 monolayers. VI. Studies of enzyme substitution therapy for phenylketonuria—a new application of Caco-2 monolayers

Autor: Sven Frokjaer, Steffen W Jorgensen, Helle Brøndsted, Lars Hovgaard, Peter Eigtved
Rok vydání: 1998
Předmět:
Zdroj: International Journal of Pharmaceutics. 161:109-114
ISSN: 0378-5173
DOI: 10.1016/s0378-5173(97)00335-9
Popis: Phenylalanine ammonia-lyase (PAL) which is an enzyme capable of converting l -phenylalanine to trans -cinnamic acid has been shown to be able to minimize the amount of l -phenylalanine transported across Caco-2 monolayers when administered apically. The impact of this is that a formulation of PAL, able to lower the transport of l -phenylalanine across epithelia, potentially could be applied in the oral treatment of phenylketonuria. Confluent monolayers of Caco-2 cells were shown to simplify the studying of the simultaneous events of conversion and transport across an epithelial barrier.
Databáze: OpenAIRE