Comparison of Different Methods for the Detection of Silica Inclusions in Plant Tissues

Autor:	Ralf Thomann, Robin Seidel, Thomas Speck, Inga C. Blecher
Rok vydání:	2012
Předmět:	Chromatography Resolution (mass spectrometry) Raman imaging Mineralogy Miscanthus sinensis Plant Science Biology biology.organism_classification Plant tissue Staining Microscopy Botany Plant species Silica coating Ecology Evolution Behavior and Systematics
Zdroj:	International Journal of Plant Sciences. 173:229-238
ISSN:	1537-5315 1058-5893
Popis:	There is no standardized method to detect silica depositions in plant tissues. Therefore, we compared current methods to reveal their advantages and disadvantages for selecting the most suitable method. We studied three plant species (Equisetum hyemale, Carex pendula, and Miscanthus sinensis) with pronounced and well-defined silica inclusions of different forms. We used two SEM methods, two stainings for light microscopy, and Raman imaging. Our results showed that energy-dispersive x-ray (EDX) analysis is highly specific but has a lower resolution than low-voltage–high-contrast (vCD) detection, which gives good resolution and a broad scanning area. In addition to the nonspecificity of vCD detection, it can be difficult to identify silica coatings with this method because of a lack of contrast with non-silica-coated tissues. Therefore, confirmation of silica detection with EDX is needed. In all plants tested, staining proved to be not silica specific but did allow for an indication of the position of silic...
Databáze:	OpenAIRE
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