DNA Damage Effect of Botanical Insecticides Using Chinese Hamster Lung Cells

Autor:	Kyongmi Chon, Kyung-Hun Park, Nam-Jun Cho, Mi-Hye Jeong, Areumnuri Kim, Min Kyoung Paik
Rok vydání:	2015
Předmět:	Gel electrophoresis DNA damage food and beverages General Medicine Biology Azadirachta medicine.disease_cause biology.organism_classification Molecular biology Chinese hamster Comet assay Clastogen Botany Toxicity medicine Genotoxicity
Zdroj:	Korean Journal of Environmental Agriculture. 34:350-354
ISSN:	2233-4173 1225-3537
DOI:	10.5338/kjea.2015.34.4.40
Popis:	BACKGROUND: Botanical insecticides, especially Azadirachta Indica extract (AIE) and Sophorae radix extract (SRE) are widely used in Agriculture field. In our previous studies on genotoxicity test of AIE and SRE samples, a suspicious clastogenic properties was shown. Herein, we investigated the DNA damage effect of these botanical insecticide samples through the in vitro comet assay. METHODS AND RESULTS: Chinese hamster lung (CHL) fibroblast cell line was used, and methyl methanesulphonate was as positive control. Respective two samples of AIE and SRE were evaluated using Single Cell Gel Electrophoresis (Comet) assay and measured as the Olive tail moment (OTM). Results from this study indicated that all tested AIE and SRE samples did not show DNA damage in comet assay using CHL cells, compared with control. CONCLUSION: AIE and SRE samples used in this study were not cause genetic toxicity and are suitable for use as organic materials.
Databáze:	OpenAIRE
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