Antifungal activity of crude extract produced byBacillussp. associated with entomopathogenic nematode from media formulated by six nitrogen sources with fructose againstPenicillium expansum

Autor: Sasidharan Kumar Nishanth, Bala Nambisan, Chellapan Mohandas
Rok vydání: 2013
Předmět:
Zdroj: Archives Of Phytopathology And Plant Protection. 46:1222-1229
ISSN: 1477-2906
0323-5408
DOI: 10.1080/03235408.2013.763616
Popis: A specific symbiotic Bacillus species isolated from a rhabditid entomopathogenic nematode, Rhabditis (Oscheius) sp. was found to produce a number of bioactive compounds. The present study was conducted to determine the effect of six different nitrogen sources in combination with fructose on the production of antifungal crude extract by Bacillus sp. against Penicillium expansum. The yield of crude extract and antifungal activity against the test fungi differed significantly when the nitrogen sources in the fermentation media were changed. The highest yield was recorded for beef extract plus fructose (921 mg/L). The antifungal activity was higher in yeast extract plus fructose [P. expansum (46.5 ± 2.12 mm)], followed by beef extract. High performance liquid chromatography analysis of the crude antimicrobial substances revealed different peaks with different retention times indicating that they produced different compounds. When a carbon source was not included in the fermentation medium, the antimicrobial p...
Databáze: OpenAIRE