Selective Determination of Iron Ion in Tap Water by Solvent Extraction with 3,4-Dihydro-3-hydroxy- 4-oxo-1,2,3-benzotriazine, Followed by Reversed Phase HPLC

Autor:	Susumu Ichinoki, Shogo Fujita, Youichi Fujii
Rok vydání:	2008
Předmět:	Detection limit Chloroform Chromatography Metal ions in aqueous solution Clinical Biochemistry Pharmaceutical Science Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Tap water Methanol
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 32:281-292
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826070802603377
Popis:	A selective determination method for Iron (Fe) ion in tap water has been developed by solvent extraction, followed by reversed phase HPLC with photometric detection. The Fe(III) ion was quantitatively extracted into chloroform over the pH range of 3.2 to 4.3 as 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (DHOB) chelate. Job's method indicated that the Fe-DHOB chelate composition was Fe(DHOB)3. The molar absorptivity of the Fe-DHOB chelate was calculated as 7.6 × 103 at 430 nm. The extracted Fe-DHOB chelate was then separated on a phenyl column with an eluent of methanol/water/0.05 M DHOB (40:20:40, v/v) and detected at 500 nm. The correlation coefficients of the calibration curves obtained with 5 mL Fe standards were about 0.999 over the range of 10 ng/mL (ppb) to 10 µg/mL (ppm). The detection limit of the Fe ion in 5 mL water was estimated as 7 ppb, which corresponded to 3 times the standard deviation of the blank peak area. Effects of foreign ions on the determination of 0.2 ppm Fe ion were...
Databáze:	OpenAIRE
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