Expression of the chemically inducible maize GST-27 promoter in potato

Autor:	Jacqueline Ann Mary Paine, Ian Jepson, Uwe Sonnewald, Nicola Mary Robertson
Rok vydání:	2000
Předmět:	Genetics Reporter gene Transgene fungi Genetic transfer food and beverages Promoter Genetically modified crops Beta-glucuronidase Biology Molecular biology chemistry.chemical_compound chemistry Gene expression Herbicide safener Agronomy and Crop Science Food Science
Zdroj:	Potato Research. 43:335-345
ISSN:	1871-4528 0014-3065
DOI:	10.1007/bf02360539
Popis:	Chemically inducible gene regulation systems provide a mechanism for a temporal control of expression of transgenes. In this study expression from the herbicide safener inducible maize GST-27 promoter was tested in potato, with the aim of using this to provide inducible expression of transgenes implicated in dormancy control. A binary vector comprising 3.8 kb of the GST-27 promoter was fused to the β-glucuronidase (GUS) reporter gene and transformed into potato. Application of the chemical inducer elevated the expression of GUS up to 40 fold in leaf tissue. However in stems, roots and tubers the GST-27 promoter caused high levels of expression of GUS in the absence of safener, demonstrating that in these tissues it acts as a constitutive promoter. A deleted promoter region of the GST-27 promoter displayed a similar expression pattern. Analysis of GUS activity in dormant and sprouting tubers showed that the GST-27 promoter was a strong constitutive promoter throughout the tuber life cycle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52f8746b4d7907fa8c2c7774c9e21b63 https://doi.org/10.1007/bf02360539 Zobrazit plný text záznamu Full text from SpringerLink